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Life Under Pressure
Researchers zoom in on how biological enzymes function in extreme pressures

Washington, D.C. — Life can thrive in some of the most extreme environments on the planet. Microbes flourish inside hot geothermal vents, beneath the frigid ice covering Antarctica and under immense pressures at the bottom of the ocean. For these organisms to survive and function, so must the enzymes that enable them to live and grow. Now, researchers from Georgetown University have homed in on what allows particular enzymes to function under extreme pressures.

Enzymes are proteins that speed up critical biochemical reactions in an organism. For an enzyme to work, its molecular structure has to be stable and flexible. To facilitate a reaction, an enzyme might have to assume different shapes, known as conformations, such as an open or closed conformation — a mechanical motion that would fail if its molecular structure were too loose or too tight.

"The overall shape of the protein has to be maintained, otherwise it will unfold and do nothing, but it also needs to be flexible enough so this shape can morph into different functional conformations," said Qi Huang, a graduate student in Toshiko Ichiye's lab at Georgetown University.

Higher temperatures would loosen the atomic interactions in an enzyme, making it less stable but more flexible. High pressures would compress the enzyme and force it to become more rigid, making it more stable but less flexible. So for an enzyme under extreme conditions to function, it must adapt to have the right level of stability and flexibility. An enzyme adapted to high pressures, for example, might be more flexible than if it were adapted to normal pressures.

To better understand the effect of pressure on an enzyme — and, in particular, how it affects its flexibility — researchers led by Ichiye used computers to simulate the behavior of an enzyme at the molecular level, under various pressures and temperatures.

The researchers wanted to know which aspect of an enzyme's flexibility enables it to function under high pressures. An enzyme could be more flexible overall, like a bowl of jelly, or more flexible only at joints, like a robot. More likely, it could be flexible in both ways.

The researchers focused on a well-studied enzyme called dihydrofolate reductase, which is found in the familiar E. coli, a bacterium that lives under normal conditions, called a mesophile. They also studied a high-pressure version of the enzyme found in M. profunda, a microbe found at the bottom of the Atlantic, making it both a piezophilic (pressure-loving) organism as well as a psychrophilic (cold-loving) organism.

By comparing the two microbes, the researchers discovered that it's the collective motion involving small groups of atoms that bear on the jellylike nature of the protein that was most important. A mesophilic enzyme should work best under normal pressures while a piezophilic enzyme should work best under high pressures. The researchers found that when these enzymes worked their best, their collective motions were similar and in order for a piezophilic enzyme to adapt to high pressures, it needed to adjust its collective motion to match a mesophile's motion under normal pressure.

Understanding how these so-called extremophiles thrive helps scientists gauge under what conditions life can exist — whether it's in the ocean, deep underground, or even outer space, Huang said.

These kinds of studies could even help researchers engineer proteins from mesophilic organisms to work in extreme conditions. "We can change the DNA sequence or the amino acids of a mesophilic protein and make it function under high pressure, low or high temperatures, just like those extremophiles," Huang said. That could be useful in industrial settings, for example in making biofuels and other chemicals that require extreme conditions for optimal production. Knowing the limits of microbial life could also be useful for sterilizing and preserving food by high-pressure processing.

Source: Biophysical Society

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APBN Editorial Calendar 2018
Obesity / Outlook for 2018
Searching for the fountain of youth
Women in Science - Making a difference
Digestive health in the 21st century - Trust your guts
Dental health - The root to good health
Cancer - Therapies and strategies for better patient outcomes
Water management / Vaccination
Regenerative medicine / Biotech start ups
Digital healthcare / 3D printing
Bones / Breast cancer
Liver health / Top science research nations & institutions
AIDS / Breakthrough of the year/Emerging trends
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