Fagopyrum tataricum, cultivated tartary buckwheat, is an important part of human diet in the high mountain areas of western China and in the Himalayan hills. It has been consumed all around the world as a potential “functional food” material, particularly with respect to their seeds and seed sprouts due to the abundance of phenolic compounds, high quality proteins and a well-balanced amount of essential amino acids and minerals.
Tartary buckwheat is rich in flavonoids compounds which have functions of lowering the blood sugar level and the blood fat and can be used to prevent and assist treating diseases such as diabetes mellitus, hyperlipidemia and high pressure.
However, the breeding program for buckwheat is very slow because of the difficult hybridization of buckwheat flower. As a result, transformation becomes an important practical method for buckwheat genetic improving. So, establishing the in vitro regeneration system is most important work for buckwheat improving.
Prof. Wang Tao’s group of Chengdu Institute of Biology, Chinese Academy of Sciences has long been focused on improving the quality improving of buckwheat, especially the genetic transforming. Recently, Prof. Wang’s group has established the in vitro regeneration system for tartary buckwheat.
This work details in the characterization of seedling age, hypocotyl and cotyledon as explants, and proportions of several growth regulators for establishing Fagopyrum tataricum Gaertn. in vitro regeneration system. The author suggested the best seedling age for callus induction was 6 to 8 days and the highest induction rate of calli from hypocotyls of MS medium containing 2.0 mg/L 2, 4-D and 1.5 mg/L 6-BA.
Furthermore, researchers also explored the best shooting medium for generation was the MS medium+0.1 mg/L IAA+2.0 mg/L 6-BA+1.0 mg/L KT+0.5 mg/L TDZ (thidiazuron). The callus induction and shooting rates were higher from hypocotyls than from cotyledons. For most, TDZ could accelerate shoot differentiation distinctively, improving the shooting rate by nearly 20%.
This work provides new sight for the genetic improving of tartary buckwheat, which now difficult for regular breeding. As a new method, this in vitro regeneration system may reduce the breeding cycle.
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