HOME ABOUT CONTACT AVAILABLE ISSUES SUBSCRIBE MEDIA & ADS CONFERENCE CALENDAR
LATEST UPDATES » Vol 21, No 05, May 2017 – Insights Into the Brain & microRNAs       » Omega-3 Therapy May Help Reverse Type 1 Diabetes       » The Potential of Saving Human Lives with Hibernation       » Scientists Unlock TCM Drug's Role in Weight Loss       » Medical Reforms in Beijing       » Fruits and Vegetables' Latest Superpower? Lowering Blood Pressure      
EYE on CHINA
Diamonds are for finding stem cells
Stem cell therapy has the potential to repair and regenerate damaged tissues. Unlocking this potential requires effective methods both for isolating and expanding stem cells and for tracking their ultimate fate when introduced into their host. A study published online in the high impact journal Nature Nanotechnology describes new methods for isolating, expanding, and labeling lung stem cells, that uses fluorescent nanodiamonds to track the fate of individual cells. This technology may offer insights into the factors that determine the acceptance of transplanted stem cells and their ability to regenerate within a host.

The labs, headed by John Yu, M.D., Ph.D., Distinguished Chair of Institute of Cellular & Organismic Biology, and Huan-Cheng Chang, Ph.D., Distinguished Research Fellow, Institute of Atomic and Molecular Sciences, Academia Sinica, collaborated on the work.

First, they developed a new stem cell isolation method for lung stem cells (LSCs), using cell surface glycoprotein markers discovered by specialized proteomic analysis. They demonstrated that cells isolated with this method possess not only the abilities to self-renew and differentiate into lung tissues, but also the in vivo homing and regenerative abilities expected of a LSC.  Next, they used fluorescent nanodiamonds (FNDs) to label these cells. FNDs are novel imaging probes which are not only chemically robust and fluorescently stable, but also biologically inert and nontoxic. These nanoparticles can be readily taken up by cells through endocytosis. When excited by green-yellow light, the built-in fluorophores emit stable far-red emission with a fluorescence lifetime of up to 15 ns, much longer than 1 ~ 4 ns of endogenous and exogenous fluorophores commonly used in cell biology. This property makes it possible to separate FND emission from strong autofluorescence backgrounds of host tissue, using special strategies.

The authors successfully demonstrated the use of this system to isolate LSCs, engraft them in mouse models of lung injury, and track single cell fates. They illustrated that FND-labeled LSCs preferentially home in to injured lungs more rapidly than to uninjured controls, enabling the lung epithelium to be restored more rapidly. In addition to demonstrating the therapeutic potential of such treatment, these results also support an “active homing” model in which transplanted LSCs proactively migrate to injured tissue, as opposed to non-specific/passive entrapment.

Yu and Chang suggested that this method has broad applicability to stem cell research, and that similar techniques could be used to monitor the uptake of different kinds of stem cells in other tissues.

This team includes PhD students Tsai-Jung Wu from Institute of Biochemistry and Molecular Biology, National Yang-Ming University, and Yan-Kai Tzeng at Department of Chemistry, National Taiwan University, Taiwan.

Source: Kim Alaniz/Flickr/CC

Click here for the complete issue.

NEWS CRUNCH  
news Healthcare Sustainability in Asia: Now is the Time to Act — FT Asia Healthcare & Life Sciences Summit 2017
news Philip Morris International and British American Tobacco receive award from PETA science group for AOP developments
news 6th Asia-Pacific Breast Cancer Summit to take place in Hong Kong
PR NEWSWIRE  
Asia Pacific Biotech News
EDITORS' CHOICE  

Lady Ganga: Nilza'S Story
COLUMNS  
Subscribe to APBN E-Newsletter
Find us under 'Others' option to receive APBN e-newsletters thrice a month!

APBN Editorial Calendar 2017
January:
Healthcare Focus: LUNGS
February:
War on CANCER
March:
Get to Know TCM
April:
Diabetes: The Big Picture
May:
The Piece of Your Mind - Brain Health/Science
June:
Rare Diseases
July:
Food Science & Technology
August:
Eye Care/ Eye Health
September:
No. 1 Killer - Heart Diseases, Diagnosis and Treatment
October:
Skin Diseases/Allergic Reactions
November:
Diseases threatening our Children
December:
Liver Health & Treatment/Technology
Editorial calendar is subjected to changes.
– Editor: Carmen, Jia Wen Loh
MAGAZINE TAGS
About Us
Events
Available issues
Editorial Board
Letters to Editor
Instructions to Authors
Advertise with Us
CONTACT
World Scientific Publishing Co. Pte. Ltd.
5 Toh Tuck Link, Singapore 596224
Tel: 65-6466-5775
Fax: 65-6467-7667
» For Editorial Enquiries:
   biotech_edit@wspc.com or Ms Carmen
» For Subscriptions, Advertisements &
   Media Partnerships Enquiries:
   biotech_ad@wspc.com
Copyright© 2017 World Scientific Publishing Co Pte Ltd  •  Privacy Policy